Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172006_fnuo TGGTAGCCTGTGGTCAGGCAGCCCTTGTAATG(+AGGACA)GATCCCTACCTTCTTGTAAAGTCTCTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613172004_0n9w ACACATTTTTAGAGACTTTACAAGAAGGTAGG(+AGGACA)GATCCATTACAAGGGCTGCCTGACCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613172003_v0s9 AATACCCTCTTTCTGACCTCCACAGGCACAGG(+AGGACA)GTACCCATGTGGTATACATATATACACAAGG NGG 10 8 17 8 24 0 100 5 10
    240613172001_573u TTTTGCCTTGTGTATATATGTATACCACATGG(+AGGACA)GTACCCTGTGCCTGTGGAGGTCAGAAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613171958_lhvh AAGGTGGCTGGCTGGGGAATCCATGGGGGGGG(+AGGACA)GATCCTGATTATAGATACTTCTGTTAGGGAT NGG 10 8 17 8 24 0 100 5 10
    240613171957_liap GCTTTATCCCTAACAGAAGTATCTATAATCAG(+AGGACA)GATCCCCCCCCCATGGATTCCCCAGCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613171956_ujyh CTGTAGATAAAACAAAATTATACCGAGTAAAG(+AGGACA)GTACCAGGATATCTGGTCCCCAAGGCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240613171954_b9hx CCTGGTTCCAGCCTTGGGGACCAGATATCCTG(+AGGACA)GTACCTTTACTCGGTATAATTTTGTTTTATC NGG 10 8 17 8 24 0 100 5 10
    240613171952_cxy0 GGCTGTTCTGCATGGGAGGAAGTGGGTTAGGG(+AGGACA)GATCCAGGCTCTTGGCCAGACATACAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613171950_c8on ATGTCAGCTCTGTATGTCTGGCCAAGAGCCTG(+AGGACA)GATCCCCTAACCCACTTCCTCCCATGCAGAA NGG 10 8 17 8 24 0 100 5 10