Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172112_novy TGGTGAAGTCCTAAGCATGACTTTAAAACCTG(+AGGACA)GATCCAATTTCAAGGGGCTCATGATAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613172111_r3dt ATATCCCACTTATCATGAGCCCCTTGAAATTG(+AGGACA)GATCCAGGTTTTAAAGTCATGCTTAGGACTT NGG 10 8 17 8 24 0 100 5 10
    240613172109_nc2l AATCTCTGCATGAGTGAAGGGAGGACACATGG(+AGGACA)GTACCGGAATGGGTCTCACAGAAATGGACTT NGG 10 8 17 8 24 0 100 5 10
    240613172107_kw9p AAAGAAAGTCCATTTCTGTGAGACCCATTCCG(+AGGACA)GTACCCATGTGTCCTCCCTTCACTCATGCAG NGG 10 8 17 8 24 0 100 5 10
    240613172106_3g1f AGGCTCCACACACCAGGTATTGCTGATTCCAG(+AGGACA)GTACCACCCTTGACATGTGTGTCTGAGTCCC NGG 10 8 17 8 24 0 100 5 10
    240613172104_39tc AGGCTGGGACTCAGACACACATGTCAAGGGTG(+AGGACA)GTACCTGGAATCAGCAATACCTGGTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240613172102_ixsn CAGCTTTGTCAACAGTTCTGTATCCATACCTG(+AGGACA)GTACCCTCGGCCCCGCTTCCGAGTCGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613172100_wqko AAGTGAAAAGCGACTCGGAAGCGGGGCCGAGG(+AGGACA)GTACCAGGTATGGATACAGAACTGTTGACAA NGG 10 8 17 8 24 0 100 5 10
    240613172058_rsxp AGAAGACACCCAGGGTGACGTCTCATCCTCAG(+AGGACA)GATCCCCCACGTCTGCTGCCATCACAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240613172056_doeg GGGATGCGGCTGTGATGGCAGCAGACGTGGGG(+AGGACA)GATCCTGAGGATGAGACGTCACCCTGGGTGT NGG 10 8 17 8 24 0 100 5 10