Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172022_h5qm GCTGCCTGAGGGAGCCTTTGGAGAGCTAACTG(+AGGACA)GTACCCTGATTATAGTCCATTAAGAATCACT NGG 10 8 17 8 24 0 100 5 10
    240613172020_15w2 ACCTAAGTGATTCTTAATGGACTATAATCAGG(+AGGACA)GTACCAGTTAGCTCTCCAAAGGCTCCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613172019_nqym AGACACAGGTGCAGAGCGGGAAGGCTGACTGG(+AGGACA)GATCCCGCCACCAGCTGTTTTCCCTCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613172017_4k08 GAAATGACAAGAGGGAAAACAGCTGGTGGCGG(+AGGACA)GATCCCAGTCAGCCTTCCCGCTCTGCACCTG NGG 10 8 17 8 24 0 100 5 10
    240613172015_gukr GGGTGGCCAGGAGCAGCAGAGGTGAAGGGATG(+AGGACA)GATCCACCTGCGGGTCTCTGTGGTTACCGTA NGG 10 8 17 8 24 0 100 5 10
    240613172013_xsue ATCACTACGGTAACCACAGAGACCCGCAGGTG(+AGGACA)GATCCATCCCTTCACCTCTGCTGCTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613172012_o0g0 CTCTGCATCCATCTTGATTAAAGATGGGTGTG(+AGGACA)GATCCTCTCCCACATCCTAGGGTGTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613172011_9m2h ATGGCTGCCCCACACCCTAGGATGTGGGAGAG(+AGGACA)GATCCACACCCATCTTTAATCAAGATGGATG NGG 10 8 17 8 24 0 100 5 10
    240613172009_76se CTCCCTTCAGCACGGTAGGTCTTTGCATCTAG(+AGGACA)GATCCCCGCCCCAGACCGCCTGTGAGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613172007_vqsr CTTTGCTCTGCTCACAGGCGGTCTGGGGCGGG(+AGGACA)GATCCTAGATGCAAAGACCTACCGTGCTGAA NGG 10 8 17 8 24 0 100 5 10