Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171917_ym3i GGGCTGTCTCTGACCCTATTACCTGCCTTTGG(+AGGACA)GATCCATTCCCCCTCCTGCATCGTCCAACAT NGG 10 8 17 8 24 0 100 5 10
    240613171915_qt42 TACTAATGTTGGACGATGCAGGAGGGGGAATG(+AGGACA)GATCCCAAAGGCAGGTAATAGGGTCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613171914_tnr6 GCCCTCTTTCCTTGGCACATTCCATGTAGTTG(+AGGACA)GTACCCTTGTGGGGACCCACAGCATCCATGG NGG 10 8 17 8 24 0 100 5 10
    240613171912_diis GGTTTCCATGGATGCTGTGGGTCCCCACAAGG(+AGGACA)GTACCAACTACATGGAATGTGCCAAGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613171911_zpva ATGCAGGTTAAGTGCCTAAGGTTTCCTGTTGG(+AGGACA)GTACCACATGCTTCTCTAAAAGGCTTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240613171909_hpfl GACTCCCTTAAAGCCTTTTAGAGAAGCATGTG(+AGGACA)GTACCCAACAGGAAACCTTAGGCACTTAACC NGG 10 8 17 8 24 0 100 5 10
    240613171908_vqsx CACTAACTACTGTGACAAGAAACTTAGCAAAG(+AGGACA)GTACCTGTGTGTACAGTGAGGGCAGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613171907_p7r7 CTCATAGCCAGCTGCCCTCACTGTACACACAG(+AGGACA)GTACCTTTGCTAAGTTTCTTGTCACAGTAGT NGG 10 8 17 8 24 0 100 5 10
    240613171906_qdul AGTCGTCCCTCTGTCAGCTTGGAGCGTCTCAG(+AGGACA)GTACCCAGGCAGTCTCAGACTTCTGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613171903_ti8r TTTCGGAGGCCCAGAAGTCTGAGACTGCCTGG(+AGGACA)GTACCTGAGACGCTCCAAGCTGACAGAGGGA NGG 10 8 17 8 24 0 100 5 10