Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171812_jb7c GGTGTCATTGCTTGGAGGCTAATTATGGGATG(+AGGACA)GATCCCTGGATATGGCAGTCTCTAGATGGAC NGG 10 8 17 8 24 0 100 5 10
    240613171810_9olu GGATGGTCCATCTAGAGACTGCCATATCCAGG(+AGGACA)GATCCATCCCATAATTAGCCTCCAAGCAATG NGG 10 8 17 8 24 0 100 5 10
    240613171808_l004 GGTGCACAGGCCTCGGAAGGCAGAGGCAGGGG(+AGGACA)GATCCCTACCGGTTTAGAATCTGCATGGGCT NGG 10 8 17 8 24 0 100 5 10
    240613171806_tqkn TATGCAGCCCATGCAGATTCTAAACCGGTAGG(+AGGACA)GATCCCCCTGCCTCTGCCTTCCGAGGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613171804_8lo6 TGTCTCGGTCCCGGCCGCTGCTCTCCAGCCAG(+AGGACA)GTACCCTCCTCACTCCCTGCTCGCGCAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613171802_ijj4 AGTGCGAACTGCGCGAGCAGGGAGTGAGGAGG(+AGGACA)GTACCTGGCTGGAGAGCAGCGGCCGGGACCG NGG 10 8 17 8 24 0 100 5 10
    240613171801_tuzt GGCGCCGGGGGCGGTGCCGGCTCCAATCACGG(+AGGACA)GATCCGCCCAAGGGCCCCAACGCGGTAGGCG NGG 10 8 17 8 24 0 100 5 10
    240613171759_bjf2 GGAAGCGCCTACCGCGTTGGGGCCCTTGGGCG(+AGGACA)GATCCCGTGATTGGAGCCGGCACCGCCCCCG NGG 10 8 17 8 24 0 100 5 10
    240613171757_shhs TCCTTCTTGCTCCGCTTCTATTCTGTCAAGGG(+AGGACA)GATCCCTGCAGTCTGAGGCTTCCCCCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240613171755_fsay CCAGGTTTGGGGGGGAAGCCTCAGACTGCAGG(+AGGACA)GATCCCCTTGACAGAATAGAAGCGGAGCAAG NGG 10 8 17 8 24 0 100 5 10