Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171741_i7hu GGACAAAGTCGCCTAATGTCTTAGTAGACAGG(+AGGACA)GTACCCCTCACCACATCCTCTTCTTAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240613171739_6674 GGCAATGACCTAAGAAGAGGATGTGGTGAGGG(+AGGACA)GTACCCTGTCTACTAAGACATTAGGCGACTT NGG 10 8 17 8 24 0 100 5 10
    240613171738_438b CCCGCCCGCCCGCCATCTATTTAGCACGTGAG(+AGGACA)GATCCCCTTCGGGTTTACTTTCACTCTCTTC NGG 10 8 17 8 24 0 100 5 10
    240613171736_wq9e ATTGTGAAGAGAGTGAAAGTAAACCCGAAGGG(+AGGACA)GATCCTCACGTGCTAAATAGATGGCGGGCGG NGG 10 8 17 8 24 0 100 5 10
    240613171735_azlv CTAGCTCCAGACCCTTCTCTACAGATGTTGTG(+AGGACA)GTACCCTCCAGCCTCCAAGCCACACAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240613171732_figx TGTGCCTGACTGTGTGGCTTGGAGGCTGGAGG(+AGGACA)GTACCACAACATCTGTAGAGAAGGGTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613171730_9jqv TTCCCCACTTCCTCACAAAGCCCTGACCCCAG(+AGGACA)GATCCCAGCGATGGACAGGAACACCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613171728_yvta GTAGACTCTGAGGTGTTCCTGTCCATCGCTGG(+AGGACA)GATCCTGGGGTCAGGGCTTTGTGAGGAAGTG NGG 10 8 17 8 24 0 100 5 10
    240613171727_utrr TGGCATGGTCATGTGCCCTCTGGCCTGTGGAG(+AGGACA)GTACCTATAGTGGCAAGGACAGGGGTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613171725_1p8t CTCCCACCTGACCCCTGTCCTTGCCACTATAG(+AGGACA)GTACCTCCACAGGCCAGAGGGCACATGACCA NGG 10 8 17 8 24 0 100 5 10