Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171634_h5uu TGTTGCTTTCGGGACCCAAAGACTCTTCCGGG(+AGGACA)GATCCAGCATCTTGCCTTCCATTCTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613171633_y6n9 CAGGCAGAAAGAGAATGGAAGGCAAGATGCTG(+AGGACA)GATCCCCGGAAGAGTCTTTGGGTCCCGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613171631_lvji CACAGATGAGAATCTAACTTGGACCCCACCTG(+AGGACA)GTACCCCACCTGGTACACTGCACATCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613171629_lzpv AAAGGACACTGATGTGCAGTGTACCAGGTGGG(+AGGACA)GTACCAGGTGGGGTCCAAGTTAGATTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240613171627_1l8j TAGTGGGTCATGGGCTCAGGTCCTTATCCCAG(+AGGACA)GATCCTCTAAGCCTTAGGTGTTGTGGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240613171625_1jzb CAGACTCTTGCCACAACACCTAAGGCTTAGAG(+AGGACA)GATCCTGGGATAAGGACCTGAGCCCATGACC NGG 10 8 17 8 24 0 100 5 10
    240613171624_gn4m AAAAGGAGTCATTCCACAATGTCTGTATGAGG(+AGGACA)GATCCAAACTCTGTCCTCTTTAATAAAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613171623_dho1 TATTCTGACTTTATTAAAGAGGACAGAGTTTG(+AGGACA)GATCCCTCATACAGACATTGTGGAATGACTC NGG 10 8 17 8 24 0 100 5 10
    240613171621_byn6 CAGTAGAACAGCACTGCTTGAGGCTCACATGG(+AGGACA)GTACCAAATCCCTCTATTTCCTGGCTTACTG NGG 10 8 17 8 24 0 100 5 10
    240613171620_a6wq CAGGACAGTAAGCCAGGAAATAGAGGGATTTG(+AGGACA)GTACCCATGTGAGCCTCAAGCAGTGCTGTTC NGG 10 8 17 8 24 0 100 5 10