Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171652_dd41 TCAGTCGGTCCCTCAACCCACGGGAAAATCAG(+AGGACA)GATCCCTGTACTCAAGTGGGCAAGGAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240613171650_94wg TTTTGCTGACTCCTTGCCCACTTGAGTACAGG(+AGGACA)GATCCTGATTTTCCCGTGGGTTGAGGGACCG NGG 10 8 17 8 24 0 100 5 10
    240613171648_ag6p CATAAATGTGCCTGTCAGGTATTGGGGAAGGG(+AGGACA)GTACCCAGTGCCCTTCCTTCTGCCTACTAAA NGG 10 8 17 8 24 0 100 5 10
    240613171646_06ev CTTACTTTAGTAGGCAGAAGGAAGGGCACTGG(+AGGACA)GTACCCCTTCCCCAATACCTGACAGGCACAT NGG 10 8 17 8 24 0 100 5 10
    240613171645_g0og TATCATAGAAGCTAGGTCTGAATCAAATCTTG(+AGGACA)GTACCTCAGAGGAAGTAGGAAGCGCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240613171644_quum CAATCCAGTGGGCGCTTCCTACTTCCTCTGAG(+AGGACA)GTACCAAGATTTGATTCAGACCTAGCTTCTA NGG 10 8 17 8 24 0 100 5 10
    240613171642_jltw TTGCCTCCCAAGGGCTAATGTTAAAGGTGTGG(+AGGACA)GTACCATCACTCCCAGGCAAATCTTCCATTT NGG 10 8 17 8 24 0 100 5 10
    240613171641_w1te TCCAAAAATGGAAGATTTGCCTGGGAGTGATG(+AGGACA)GTACCCACACCTTTAACATTAGCCCTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613171639_er15 CTATGGGCCCTTTGGTGACATGTGGGGCTCTG(+AGGACA)GATCCTTGGGTGTCCTCACTGTGGCCACTCT NGG 10 8 17 8 24 0 100 5 10
    240613171637_ea0h CAGGCAGAGTGGCCACAGTGAGGACACCCAAG(+AGGACA)GATCCAGAGCCCCACATGTCACCAAAGGGCC NGG 10 8 17 8 24 0 100 5 10