Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171600_aywf CCTTTGAGACAGAGCAATGATCTAAGACATAG(+AGGACA)GTACCTCCATTGGGCAGGTCCCCCAGTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613171559_0803 GCCAGATCAACTGGGGGACCTGCCCAATGGAG(+AGGACA)GTACCTATGTCTTAGATCATTGCTCTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613171557_8b7e AGGCCATGTTCCTGTGGGAGGGGCTGTGGAAG(+AGGACA)GATCCACACCGGGCTGTTTCCATCTGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613171556_koux GCTTCGTTTCCAGATGGAAACAGCCCGGTGTG(+AGGACA)GATCCTTCCACAGCCCCTCCCACAGGAACAT NGG 10 8 17 8 24 0 100 5 10
    240613171554_p50f GTGGGACAGGCCATGATTAGGGGCACAGGGAG(+AGGACA)GATCCCAGGGCCTTAATGGAGTCAACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613171551_f0wt TGTCTCCCAGGTTGACTCCATTAAGGCCCTGG(+AGGACA)GATCCTCCCTGTGCCCCTAATCATGGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613171549_0ni9 GTTTGTTCCTCTTGTCAAGGCTTGCTCTCTGG(+AGGACA)GATCCCCTCTGCCCCAGGACAAGGGGACCTT NGG 10 8 17 8 24 0 100 5 10
    240613171548_phz3 AGGGGAAGGTCCCCTTGTCCTGGGGCAGAGGG(+AGGACA)GATCCCAGAGAGCAAGCCTTGACAAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613171545_5czt ACAGCGGGTGAGAGGTGTCTCTGTGTCTCCGG(+AGGACA)GTACCATAGGCCCTACGAGGTTGCCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240613171543_4b10 GAGGCGGTGCTGGCAACCTCGTAGGGCCTATG(+AGGACA)GTACCCGGAGACACAGAGACACCTCTCACCC NGG 10 8 17 8 24 0 100 5 10