Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171450_u4b6 CTTGTCTGCAGTCTCCACTCTGCCGGAAGTCG(+AGGACA)GATCCCCTTTTAGACTCCAGGTTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613171448_q7rv CCAGGAGAAGAGGAACCTGGAGTCTAAAAGGG(+AGGACA)GATCCGACTTCCGGCAGAGTGGAGACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613171446_clhe TCCTCCCTCTGGGTTCATCCTGACCAATCAGG(+AGGACA)GTACCCTGAGTTCCTACCCAGTCCCCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613171445_6i96 CAGTATGAAAGGGGACTGGGTAGGAACTCAGG(+AGGACA)GTACCCTGATTGGTCAGGATGAACCCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613171442_isyr CTAGCAGGGGGCTTGTACAGTGAGTGATGGGG(+AGGACA)GATCCCAGGAGAGTGCTGGTGATGGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613171440_iyh2 CTCTGACCCAGCCATCACCAGCACTCTCCTGG(+AGGACA)GATCCCCCATCACTCACTGTACAAGCCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613171439_xt2l TACATCCTCATCACCAGTGCGGCTGGGTTCTG(+AGGACA)GTACCAGCTTCAGCACCCCACAGTACCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613171438_3kol CTGAACCCTGGTACTGTGGGGTGCTGAAGCTG(+AGGACA)GTACCAGAACCCAGCCGCACTGGTGATGAGG NGG 10 8 17 8 24 0 100 5 10
    240613171436_ihot GCCGCGCCATATCTCGCGGCCAGTTCCTGCAG(+AGGACA)GTACCGGCTCGCCCTGCCAGTCGCAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240613171435_6dyd CGGGGCTGGCTTGCGACTGGCAGGGCGAGCCG(+AGGACA)GTACCTGCAGGAACTGGCCGCGAGATATGGC NGG 10 8 17 8 24 0 100 5 10