Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171433_a7gc AGACCCACCGAGGGGGAAGAGTGTGTCAAGGG(+AGGACA)GTACCTAGGAGACCTGCTAAGCCGTCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613171431_qkkc CTGTTGCTGGGACGGCTTAGCAGGTCTCCTAG(+AGGACA)GTACCCCTTGACACACTCTTCCCCCTCGGTG NGG 10 8 17 8 24 0 100 5 10
    240613171429_0iqc TTATAGTCCGTCATCCAGAGAAGTCAGGGCGG(+AGGACA)GATCCTGGAGGCAAGGACTGAAGCAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613171427_6162 TCCACAGCCTCTGCTTCAGTCCTTGCCTCCAG(+AGGACA)GATCCCGCCCTGACTTCTCTGGATGACGGAC NGG 10 8 17 8 24 0 100 5 10
    240613171426_shse CGCATCTGTAGTACCAGAACTCAAGGCAGGAG(+AGGACA)GATCCTTGCTAAGTTTGAAGCCAGCCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240613171424_u50h AAGTAGCCTAGGCTGGCTTCAAACTTAGCAAG(+AGGACA)GATCCTCCTGCCTTGAGTTCTGGTACTACAG NGG 10 8 17 8 24 0 100 5 10
    240613171423_89b5 GCCACTAGGCGAGTGGAGGAAGCTATAACCAG(+AGGACA)GATCCTGGGGGAGAGGGGCTGCCTACGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613171419_mg7z CCTTCCCTCCGTAGGCAGCCCCTCTCCCCCAG(+AGGACA)GATCCTGGTTATAGCTTCCTCCACTCGCCTA NGG 10 8 17 8 24 0 100 5 10
    240613171418_vx6h GGGGAGGAGGGGGGGGGCTGAGAAGACTCCTG(+AGGACA)GTACCCAGTGAAAACACCTAGCCATGCATGA NGG 10 8 17 8 24 0 100 5 10
    240613171416_3atj TGTTGTCATGCATGGCTAGGTGTTTTCACTGG(+AGGACA)GTACCAGGAGTCTTCTCAGCCCCCCCCCTCC NGG 10 8 17 8 24 0 100 5 10