Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171415_0fa7 TCGGATGGCAGAGCATTCTAGGAAGGGACGAG(+AGGACA)GATCCAAGTGTAGTCTGTCTTTTAAGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240613171414_lc39 TGCCCAAGACCTTAAAAGACAGACTACACTTG(+AGGACA)GATCCTCGTCCCTTCCTAGAATGCTCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240613171413_khmb AGGCCAGTCTGGTAGGCAGTTTTTCAATTGAG(+AGGACA)GATCCCTCTTCTCAGATTACTCTAGCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240613171411_uktn TTTGATACAAGCTAGAGTAATCTGAGAAGAGG(+AGGACA)GATCCTCAATTGAAAAACTGCCTACCAGACT NGG 10 8 17 8 24 0 100 5 10
    240613171410_vgjw TAATCCCAGCTCCTAGGAGGCAGGGACAAGTG(+AGGACA)GATCCCTGTGAGTTAGAGACTAGCCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240613171408_gr1x TATGTAGACCAGGCTAGTCTCTAACTCACAGG(+AGGACA)GATCCACTTGTCCCTGCCTCCTAGGAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613171407_b9ms GAAGAGAGACAAGGAGAAGCAGAAAGCAGAAG(+AGGACA)GTACCCTTTTATTGGCATTTTAACAATGGCA NGG 10 8 17 8 24 0 100 5 10
    240613171405_ah8k CGTGCTGCCATTGTTAAAATGCCAATAAAAGG(+AGGACA)GTACCTTCTGCTTTCTGCTTCTCCTTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613171404_01iv TCTTTTTGTAAGGTTCTGAGACTTTATGGAAG(+AGGACA)GTACCTCTGCATTCTCATAAACCCTGACTCT NGG 10 8 17 8 24 0 100 5 10
    240613171402_otlk GGATCAGAGTCAGGGTTTATGAGAATGCAGAG(+AGGACA)GTACCTTCCATAAAGTCTCAGAACCTTACAA NGG 10 8 17 8 24 0 100 5 10