Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171505_4ako CCCCTGGTGCTAGATGCAAGGTCAAGAGGAGG(+AGGACA)GTACCTTCTGTCAGGTATGAGCTGCCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613171504_hd17 GGATGTGGAAGGCAGCTCATACCTGACAGAAG(+AGGACA)GTACCCTCCTCTTGACCTTGCATCTAGCACC NGG 10 8 17 8 24 0 100 5 10
    240613171503_yo8e GTGTGTGTGTGTATCTGAGAATGTGGGTGTAG(+AGGACA)GTACCCTCCAAGGCCAGAAGAGGGCATCAGG NGG 10 8 17 8 24 0 100 5 10
    240613171501_fdo2 GGAGACCTGATGCCCTCTTCTGGCCTTGGAGG(+AGGACA)GTACCTACACCCACATTCTCAGATACACACA NGG 10 8 17 8 24 0 100 5 10
    240613171459_hity CCCAGGAGAAGAGGAACCTGGAGTCTAAAGGG(+AGGACA)GATCCGACTTCCGGCAGAGTGGAGACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613171458_1nlu CTTGTCTGCAGTCTCCACTCTGCCGGAAGTCG(+AGGACA)GATCCCCTTTAGACTCCAGGTTCCTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613171456_skn9 CCAGGAGAAGAGGAACCTGGAGTCTAAAAGGG(+AGGACA)GATCCGACTTCCGGCAGAGTGGAGACTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613171455_ym01 TGTCTGCAGAGTCTCCACTCTGCCGGAAGTCG(+AGGACA)GATCCCCTTTTAGACTCCAGGTTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613171454_avyb CTTGTCTGCAGTCTCCACTCTGCCGGAAGTCG(+AGGACA)GATCCCCTTTTAGACTCCAGTTTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613171452_r52a CCAGGAGAAGAGGAAACTGGAGTCTAAAAGGG(+AGGACA)GATCCGACTTCCGGCAGAGTGGAGACTGCAG NGG 10 8 17 8 24 0 100 5 10