Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171618_gokr CACCACCCCAGAGGTCAGCCTTTGGTGCATGG(+AGGACA)GATCCAAGCCAGGACAAAGCACAAGCACAGA NGG 10 8 17 8 24 0 100 5 10
    240613171617_hywi TCCAGTCTGTGCTTGTGCTTTGTCCTGGCTTG(+AGGACA)GATCCCATGCACCAAAGGCTGACCTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613171614_yn0f CATCACCCTGTGCTACCAGCATCTGGTCCCAG(+AGGACA)GATCCAGTCAGGCTAGGATTGTCAAAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613171613_6jrl CTTGTGAACTTTTGACAATCCTAGCCTGACTG(+AGGACA)GATCCTGGGACCAGATGCTGGTAGCACAGGG NGG 10 8 17 8 24 0 100 5 10
    240613171611_sbk9 GAGGGGGGTGTGAAAGCCCACAAACCTTCCTG(+AGGACA)GATCCCAGCCTCCCTTCACTGGGGTACAGAC NGG 10 8 17 8 24 0 100 5 10
    240613171609_d5v8 TGGGTGTCTGTACCCCAGTGAAGGGAGGCTGG(+AGGACA)GATCCAGGAAGGTTTGTGGGCTTTCACACCC NGG 10 8 17 8 24 0 100 5 10
    240613171608_et1q GCATGTTAATGCTCCCCCCCACCCCGGCTTTG(+AGGACA)GTACCGGGGGGGGGGGCAGGAAACTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613171605_8vxw CCCCGTGCCTCAGTTTCCTGCCCCCCCCCCCG(+AGGACA)GTACCAAAGCCGGGGTGGGGGGGAGCATTAA NGG 10 8 17 8 24 0 100 5 10
    240613171603_kl1x ACACCGTTCAATTTTGCACATATGTCCTCATG(+AGGACA)GATCCCCAGTTTTGTCCCTCCACTTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613171602_czcg CTCGTGTGCCCAAGTGGAGGGACAAAACTGGG(+AGGACA)GATCCATGAGGACATATGTGCAAAATTGAAC NGG 10 8 17 8 24 0 100 5 10