Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171542_qai2 CAGGCTTCCCCATGAGCTCTGGGCCCTCCAAG(+AGGACA)GATCCACATCCCCCAACAGCCAGTTCCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613171540_1lsj TCTGTCACTGGAACTGGCTGTTGGGGGATGTG(+AGGACA)GATCCTTGGAGGGCCCAGAGCTCATGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613171538_w8wl GAGTAAAGTTAGACCAGGACAGAGGGCCATGG(+AGGACA)GTACCTAGGTAGCCTCAGTTTCCCCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613171536_rafk AGTGACAGAGAGGGGAAACTGAGGCTACCTAG(+AGGACA)GTACCCATGGCCCTCTGTCCTGGTCTAACTT NGG 10 8 17 8 24 0 100 5 10
    240613171535_8e12 ATGGCTGTCCTGGTCCTCTCTGGACCGCTGTG(+AGGACA)GTACCAGGGTCCAGTGATGGAATGTTTATAG NGG 10 8 17 8 24 0 100 5 10
    240613171532_4kjm CAAGCCTATAAACATTCCATCACTGGACCCTG(+AGGACA)GTACCACAGCGGTCCAGAGAGGACCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240613171531_04xr TCAGGCAGAATCTGAAGAAGACACGTGGCCAG(+AGGACA)GATCCCCTGGAGGGTGCATGGGGCTGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613171528_mw5h CCCCTCTCACCAGCCCCATGCACCCTCCAGGG(+AGGACA)GATCCTGGCCACGTGTCTTCTTCAGATTCTG NGG 10 8 17 8 24 0 100 5 10
    240613171526_n48d GTCCACCTAATATAGCAGGTGGCCAGGACTGG(+AGGACA)GATCCAGCTGCCTGGATCAGGTCAGGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240613171525_akc3 CTTCCTCAAGCCTGACCTGATCCAGGCAGCTG(+AGGACA)GATCCCAGTCCTGGCCACCTGCTATATTAGG NGG 10 8 17 8 24 0 100 5 10