Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171724_lsfz CCTGGAACAGGATATCAATGAAGCAGCTGAGG(+AGGACA)GTACCAAGAGTGTGGGGTAAGGATTTCATTC NGG 10 8 17 8 24 0 100 5 10
    240613171723_y6zk CCCTTGAATGAAATCCTTACCCCACACTCTTG(+AGGACA)GTACCCTCAGCTGCTTCATTGATATCCTGTT NGG 10 8 17 8 24 0 100 5 10
    240613171720_apu9 CTGAGGAAGCTACAAAGTGTGTTCTCTCCAGG(+AGGACA)GTACCTTCCAATGCTTCCCTAGCTTCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240613171719_pw5b TGTGGGCCAAGAAGCTAGGGAAGCATTGGAAG(+AGGACA)GTACCCTGGAGAGAACACACTTTGTAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613171717_2fpy CAGTCACCCTTGGTGACATCACACTAACACGG(+AGGACA)GTACCAGAAGAGCAAGTCTGCCCGGCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613171716_dvaz ACTGGGCTCTGCCGGGCAGACTTGCTCTTCTG(+AGGACA)GTACCCGTGTTAGTGTGATGTCACCAAGGGT NGG 10 8 17 8 24 0 100 5 10
    240613171715_8fb0 CTCCTAGCAAACTTGGAGACCCCCCCAGTCTG(+AGGACA)GATCCAACTGTAACTCTCTGTGCTCCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613171713_h104 TCCAGAACCAGGAGCACAGAGAGTTACAGTTG(+AGGACA)GATCCAGACTGGGGGGGTCTCCAAGTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240613171712_xofd GCCCTTCAGTCTGCTCCTCCACGGGGTGTTCG(+AGGACA)GATCCCGATTGAGGCCCTGAATGCCCGCCAT NGG 10 8 17 8 24 0 100 5 10
    240613171710_lko0 GAGGAATGGCGGGCATTCAGGGCCTCAATCGG(+AGGACA)GATCCGAACACCCCGTGGAGGAGCAGACTGA NGG 10 8 17 8 24 0 100 5 10