Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171754_s2lr TGGGTCCCAATGCATCTGCTGGGGGCTGAGAG(+AGGACA)GTACCTTCTGCCCATCACCAGCCTTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613171753_lmrm CAGCACAGAACAAGGCTGGTGATGGGCAGAAG(+AGGACA)GTACCTCTCAGCCCCCAGCAGATGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240613171752_b956 TTCCTTTACTGAAGGAGGGCCAAGTCAGCATG(+AGGACA)GTACCACTGAGACAGATTTTTTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613171750_1uc1 TGTTTAAAAAAAAAAAAAAATCTGTCTCAGTG(+AGGACA)GTACCATGCTGACTTGGCCCTCCTTCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240613171749_yceu GCTGGAGTCTTAAAGTCAGAGGTTTCTCTTTG(+AGGACA)GATCCAATCAAGATTGAACAAATACTGAATG NGG 10 8 17 8 24 0 100 5 10
    240613171748_kp5f TGCCACATTCAGTATTTGTTCAATCTTGATTG(+AGGACA)GATCCAAAGAGAAACCTCTGACTTTAAGACT NGG 10 8 17 8 24 0 100 5 10
    240613171747_ztvt TGCCGAGTGTTTCGGATGAGAGGATTTTCATG(+AGGACA)GTACCTTCCATTTGTGTGCAAAGTTGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613171746_xp4r GGCAGCTTTCCAACTTTGCACACAAATGGAAG(+AGGACA)GTACCATGAAAATCCTCTCATCCGAAACACT NGG 10 8 17 8 24 0 100 5 10
    240613171744_7fi2 CAGCCCTCTTTCTCGGCTCATAATTAGGAGTG(+AGGACA)GTACCCTACACAATGACTGAAAACAAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613171743_7xd5 TTTACGTTCTTTGTTTTCAGTCATTGTGTAGG(+AGGACA)GTACCACTCCTAATTATGAGCCGAGAAAGAG NGG 10 8 17 8 24 0 100 5 10