Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171709_vpea TAGCCCCCAGCATCACAGAAAATGACCTAGTG(+AGGACA)GTACCCACCTGTGATCCCAGGCACCAGAAGA NGG 10 8 17 8 24 0 100 5 10
    240613171707_3dvl CCAGATCTTCTGGTGCCTGGGATCACAGGTGG(+AGGACA)GTACCACTAGGTCATTTTCTGTGATGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613171705_zoa8 TGCAGGTGGGGCCCACTAGATGGTTTCTGGGG(+AGGACA)GATCCCCAGAACATGGGGTTCACCTATCCTA NGG 10 8 17 8 24 0 100 5 10
    240613171703_6i4c GTAGATAGGATAGGTGAACCCCATGTTCTGGG(+AGGACA)GATCCCCCAGAAACCATCTAGTGGGCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613171701_pv66 GGCACTTGAGAGGACACGTGCTTTCACACAGG(+AGGACA)GTACCAAAATATCTTCTGGGGACACCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240613171700_3six AAGGTGTTGGGGTGTCCCCAGAAGATATTTTG(+AGGACA)GTACCCTGTGTGAAAGCACGTGTCCTCTCAA NGG 10 8 17 8 24 0 100 5 10
    240613171658_77r4 ACCGAGGCTGCTGAGCTCTTCCAGCATGGAGG(+AGGACA)GTACCATGGCCCGTAGGAGCCTGGGGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613171656_mojd TTGCTAGGAACCCCAGGCTCCTACGGGCCATG(+AGGACA)GTACCCTCCATGCTGGAAGAGCTCAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613171655_h9dd CAGTTGGTGAGCAGTGGCCAGTGAGCAATGAG(+AGGACA)GATCCCATGGGTGGTCAAGTGGACCTGGGGG NGG 10 8 17 8 24 0 100 5 10
    240613171653_0har AATGTCCCCCAGGTCCACTTGACCACCCATGG(+AGGACA)GATCCTCATTGCTCACTGGCCACTGCTCACC NGG 10 8 17 8 24 0 100 5 10