Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171312_424d GATAATCTATCATTTGAATGGCAAGAGACCTG(+AGGACA)GTACCCTCTATCTCGCTACTCTGGAGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240613171311_y929 CTAGGGGAGGCTCCAGAGTAGCGAGATAGAGG(+AGGACA)GTACCAGGTCTCTTGCCATTCAAATGATAGA NGG 10 8 17 8 24 0 100 5 10
    240613171308_dgog TCAACTCAGGCTCCCCATGACACTGGACCTGG(+AGGACA)GTACCACAAGAAACCGGGTGAGGCCTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240613171307_v4ry TTCCTCTCCCAGGCCTCACCCGGTTTCTTGTG(+AGGACA)GTACCCAGGTCCAGTGTCATGGGGAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613171306_9g8d GCCCACTCCTGCAGACACAGCAGATCCAGCTG(+AGGACA)GTACCCTAGCTGCCGCCCAGGGCAACCGGCC NGG 10 8 17 8 24 0 100 5 10
    240613171304_i0wh AGACTGGCCGGTTGCCCTGGGCGGCAGCTAGG(+AGGACA)GTACCAGCTGGATCTGCTGTGTCTGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613171301_j6by GGTTATCCGGGAAGTCCTGACAGGCCCCAGGG(+AGGACA)GTACCAGCTGGGAGCAGGGTAACCTGGTGTC NGG 10 8 17 8 24 0 100 5 10
    240613171300_7qgf GGAGGGACACCAGGTTACCCTGCTCCCAGCTG(+AGGACA)GTACCCCTGGGGCCTGTCAGGACTTCCCGGA NGG 10 8 17 8 24 0 100 5 10
    240613171258_ru5q AGGGCTGAGTCTGAGGCACCATCTGGCCTCTG(+AGGACA)GATCCCTGTGCCCGCCGGCCATGCCACCTCC NGG 10 8 17 8 24 0 100 5 10
    240613171257_und8 GGACAGGAGGTGGCATGGCCGGCGGGCACAGG(+AGGACA)GATCCAGAGGCCAGATGGTGCCTCAGACTCA NGG 10 8 17 8 24 0 100 5 10