Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172218_4ykw CACTTCAAGCAGAGGGAGCACTACGTGTCCTG(+AGGACA)GATCCAGAGCCAGTGGGGGACTGTTCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240613172217_i6p6 TGATGCCTCAGAACAGTCCCCCACTGGCTCTG(+AGGACA)GATCCAGGACACGTAGTGCTCCCTCTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613172215_afl8 CCACCTAAAACTCAGATTTTATACTCCTGTCG(+AGGACA)GTACCCACGTTCTATTTCTAACACATACCAA NGG 10 8 17 8 24 0 100 5 10
    240613172213_p6zj TGTCGTTGGTATGTGTTAGAAATAGAACGTGG(+AGGACA)GTACCGACAGGAGTATAAAATCTGAGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613172212_e5w8 TATTTTGACATCACCAACTCAAAATGCTAACG(+AGGACA)GTACCACTAATTGTATGCAATCTGGAATGAT NGG 10 8 17 8 24 0 100 5 10
    240613172210_0vag TTAAGATCATTCCAGATTGCATACAATTAGTG(+AGGACA)GTACCGTTAGCATTTTGAGTTGGTGATGTCA NGG 10 8 17 8 24 0 100 5 10
    240613172208_2q08 AGACTTCCCTTCTACCTCAAGTGCATTTCCAG(+AGGACA)GATCCTTTTGAAGAGAAAATTCAGAGCCTTA NGG 10 8 17 8 24 0 100 5 10
    240613172207_aj93 TAAAGTAAGGCTCTGAATTTTCTCTTCAAAAG(+AGGACA)GATCCTGGAAATGCACTTGAGGTAGAAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613172206_eqnk GGTGATTCTGCTTTAAATGAGTTCCTCAGAAG(+AGGACA)GATCCGATATCAGCTAGTTTAGGAAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240613172205_q773 AATTTCCATGCTTCCTAAACTAGCTGATATCG(+AGGACA)GATCCTTCTGAGGAACTCATTTAAAGCAGAA NGG 10 8 17 8 24 0 100 5 10