Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172310_3s82 CTGGTACCCGTGAAGGTCAGAAGAGGGTGAAG(+AGGACA)GATCCTCTGGAACTGGAGTTACCGATGGTTA NGG 10 8 17 8 24 0 100 5 10
    240613172308_vmkj GTTTATAACCATCGGTAACTCCAGTTCCAGAG(+AGGACA)GATCCTTCACCCTCTTCTGACCTTCACGGGT NGG 10 8 17 8 24 0 100 5 10
    240613172306_3d25 ACACACAGCAAACCCCACGGCTTCCCAGACGG(+AGGACA)GATCCTTCTGCTCCCTTCTACGAGGGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240613172304_pnh6 AGGTAGTCTGCCCTCGTAGAAGGGAGCAGAAG(+AGGACA)GATCCCGTCTGGGAAGCCGTGGGGTTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613172303_b2y2 ATGCTTTCTAAGGATGCTCGATAAACTTATTG(+AGGACA)GATCCCCAGAGAGCACGTTGGCAGAATCATG NGG 10 8 17 8 24 0 100 5 10
    240613172301_xtes TGAAGCATGATTCTGCCAACGTGCTCTCTGGG(+AGGACA)GATCCAATAAGTTTATCGAGCATCCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240613172300_61uu ACCTGGAATTTGCCTATGCTCAGTCTTGGATG(+AGGACA)GATCCGGAATAGTTTCATGGAACAGTGATAA NGG 10 8 17 8 24 0 100 5 10
    240613172258_n6vv GGCAATTATCACTGTTCCATGAAACTATTCCG(+AGGACA)GATCCATCCAAGACTGAGCATAGGCAAATTC NGG 10 8 17 8 24 0 100 5 10
    240613172256_bn6r CAGATCTTCCCACCTCAGGCTCCAAGTGCTGG(+AGGACA)GATCCCAACCCTAACCCTAAGGGACAGATTT NGG 10 8 17 8 24 0 100 5 10
    240613172255_nu0j GACATAAATCTGTCCCTTAGGGTTAGGGTTGG(+AGGACA)GATCCCAGCACTTGGAGCCTGAGGTGGGAAG NGG 10 8 17 8 24 0 100 5 10