Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172447_n9df AGCAACAGAAAAACAACCAAGATGTTGTGGTG(+AGGACA)GTACCTTCCTAAAATCCCGGCACTTGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613172445_0d9p CTCCACCTTCCAAGTGCCGGGATTTTAGGAAG(+AGGACA)GTACCACCACAACATCTTGGTTGTTTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613172444_geig TGGTTGCCTTCCTCCCTCGGAATGCTTGCACG(+AGGACA)GTACCTTCTAGAACCATGAACGTTAACCCCT NGG 10 8 17 8 24 0 100 5 10
    240613172443_12yq TCCCCAGGGGTTAACGTTCATGGTTCTAGAAG(+AGGACA)GTACCGTGCAAGCATTCCGAGGGAGGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613172440_3ld5 ACAAAACCAGTTTTATGGTGGTGGTGGTGGGG(+AGGACA)GTACCACCACCACATGATTATATATTGATGG NGG 10 8 17 8 24 0 100 5 10
    240613172439_6rz7 ATGAGCCATCAATATATAATCATGTGGTGGTG(+AGGACA)GTACCCCCACCACCACCACCATAAAACTGGT NGG 10 8 17 8 24 0 100 5 10
    240613172437_usse CGGCTGGTGAATGAAGAGACTAACCTATTTGG(+AGGACA)GTACCTATCATGTGTAAAGCCTGGATACATT NGG 10 8 17 8 24 0 100 5 10
    240613172436_hqrk ATGTGAATGTATCCAGGCTTTACACATGATAG(+AGGACA)GTACCCAAATAGGTTAGTCTCTTCATTCACC NGG 10 8 17 8 24 0 100 5 10
    240613172434_iuqd CCCTCTGTGCATCTGCACTACCTGTGTGTCTG(+AGGACA)GTACCCGTGAAGGTCAGAAGAGGGTGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613172433_746f GAGGATCCTTCACCCTCTTCTGACCTTCACGG(+AGGACA)GTACCAGACACACAGGTAGTGCAGATGCACA NGG 10 8 17 8 24 0 100 5 10