Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172727_8sux TCCAATCTCGGAAAACAAATCAAAATATTAAG(+AGGACA)GATCCCTGAAATATTAATCCTAATGAGATCT NGG 10 8 17 8 24 0 100 5 10
    240613172726_lq3p CAGTGAGATCTCATTAGGATTAATATTTCAGG(+AGGACA)GATCCTTAATATTTTGATTTGTTTTCCGAGA NGG 10 8 17 8 24 0 100 5 10
    240613172724_fik6 TCTGTGCTCAGGTCACACAGGGGGCTTCAGAG(+AGGACA)GATCCTTATGAAAGATGAGTTAAAAAATGTC NGG 10 8 17 8 24 0 100 5 10
    240613172723_axlu CACTTGACATTTTTTAACTCATCTTTCATAAG(+AGGACA)GATCCTCTGAAGCCCCCTGTGTGACCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613172721_z51v TTGGTGTTTCCTGACCTGATCTGCAAACTGGG(+AGGACA)GATCCCTGGTCTGTACCTGAATTCTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613172718_ue0o ATATGAAAGAAAGAATTCAGGTACAGACCAGG(+AGGACA)GATCCCCAGTTTGCAGATCAGGTCAGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613172717_uqid CCCTCACCCCAGAGTAAGCTGCACTGTTCTTG(+AGGACA)GTACCAGAGATCCGCTAGTGCACAAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240613172716_zifu TTACCGAGTCTTTGTGCACTAGCGGATCTCTG(+AGGACA)GTACCAAGAACAGTGCAGCTTACTCTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613172714_9eoo TGCAGCCGCAGCACCCGAGTCTGGAGAGAGAG(+AGGACA)GATCCCTTGAGCACAGGGATTGGAGGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613172713_lxhg CCCAGGCTGGCCTCCAATCCCTGTGCTCAAGG(+AGGACA)GATCCTCTCTCTCCAGACTCGGGTGCTGCGG NGG 10 8 17 8 24 0 100 5 10