Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173917_joow GCACGATTTTTCTTTCTCAAGGATCATGGCAG(+AGGACA)GATCCCAGAACTCTTATCCAAGTGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240613173915_0hn7 ACAGGGCCTTACTGGCTCAGGGATTTGCCTGG(+AGGACA)GTACCTATGATTTCTCTTTCCCTACCCCATG NGG 10 8 17 8 24 0 100 5 10
    240613173914_qfpq ATCAACATGGGGTAGGGAAAGAGAAATCATAG(+AGGACA)GTACCCAGGCAAATCCCTGAGCCAGTAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613173912_88mc AGGTTAGCAGTGGCAAGCTCACATAGAAGTGG(+AGGACA)GTACCACAGGGGTGAAGTAAGAGGTGCACCC NGG 10 8 17 8 24 0 100 5 10
    240613173910_c9qm TCTGTGGGTGCACCTCTTACTTCACCCCTGTG(+AGGACA)GTACCCACTTCTATGTGAGCTTGCCACTGCT NGG 10 8 17 8 24 0 100 5 10
    240613173908_a8kt CCTTAAGCTGTACTACCCTTTTCTAGACATGG(+AGGACA)GATCCAGGTGTCCTGGTATCAGAGATCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613173907_4d9t GAGGGCAAGGATCTCTGATACCAGGACACCTG(+AGGACA)GATCCCATGTCTAGAAAAGGGTAGTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240613173905_qck2 TAATCTCAGTGCTGTGGAGGCAGAGACAGGAG(+AGGACA)GATCCCTGAGCAGAAAAGCCCTACTGATGAG NGG 10 8 17 8 24 0 100 5 10
    240613173903_kuc1 TGGAGCTCATCAGTAGGGCTTTTCTGCTCAGG(+AGGACA)GATCCTCCTGTCTCTGCCTCCACAGCACTGA NGG 10 8 17 8 24 0 100 5 10
    240613173902_fd6f CTCAGGGAAGAGTGGTCACAGAGGGACAGAGG(+AGGACA)GATCCCACCCCCACCCCCACCCCCACCTGGA NGG 10 8 17 8 24 0 100 5 10