Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173845_zbpc TGAGTTCAAGGCCTGCCAGAGCAATTTAGTGG(+AGGACA)GATCCTGCCTTCAAACAAGAAGAGGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613173844_8042 GCATCAACCAGTGGGCTTCAAGCAGAGAGAGG(+AGGACA)GATCCACATTGCTGTCCTATCGACACTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613173842_c84w CAAGGCTTCAGTGTCGATAGGACAGCAATGTG(+AGGACA)GATCCCTCTCTCTGCTTGAAGCCCACTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613173841_5qma CTCCTGGAAAGCCAGAGAGAGCTACATAGTTG(+AGGACA)GATCCAGTTTCAACCCCCCCTGTTCCCTACC NGG 10 8 17 8 24 0 100 5 10
    240613173840_2rwd CCCTTGGTAGGGAACAGGGGGGGTTGAAACTG(+AGGACA)GATCCAACTATGTAGCTCTCTCTGGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613173838_eyu8 ACACCGCTAAGGTATTTTAAACCATGTTCTAG(+AGGACA)GATCCTTGAATTACCCAGTACAACATGTAAA NGG 10 8 17 8 24 0 100 5 10
    240613173837_1cwi AGCTATTTACATGTTGTACTGGGTAATTCAAG(+AGGACA)GATCCTAGAACATGGTTTAAAATACCTTAGC NGG 10 8 17 8 24 0 100 5 10
    240613173836_s4s8 CGTGTGAGACCTGGTAAGAAAACTTCCACAAG(+AGGACA)GATCCTTCCTACAGTGGAATGTCAGGCCATC NGG 10 8 17 8 24 0 100 5 10
    240613173835_q8np GCTGTGATGGCCTGACATTCCACTGTAGGAAG(+AGGACA)GATCCTTGTGGAAGTTTTCTTACCAGGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613173833_ptxa GTAAGGTGGAGTTCGAAAGAGGGGCTAGTGTG(+AGGACA)GTACCCCAAAACAACACACAACCCTGCTCTG NGG 10 8 17 8 24 0 100 5 10