Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115402_4hox CAATATAGTTTACCAAGTATGCCTTTCTCTTG(+AAAAAA)GATCCCATCTGCCTTCCAAGTGTCTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240606115401_6d06 CATCTACTTTGCCTTTTTGTGCAGGTTATATG(+AAAAAA)GATCCTGCACCGCTCATTACAAAGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606115400_c5pg AAACGTGCAGACCTTTGTAATGAGCGGTGCAG(+AAAAAA)GATCCATATAACCTGCACAAAAAGGCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606115358_09wy GAGTCCAGCATAACCAGAAAGTCCATGAGCTG(+AAAAAA)GTACCCCTGAAGCCTGGGTGTGCAGACGAGC NGG 10 8 17 8 24 0 100 5 10
    240606115357_hvfx TAGAGGCTCGTCTGCACACCCAGGCTTCAGGG(+AAAAAA)GTACCAGCTCATGGACTTTCTGGTTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606115354_2ppv TGGCCCTTGCAGTACTGTAAGATTGTGATGGG(+AAAAAA)GATCCTTTTGTTTGTTTGACTAATGTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240606115353_duel ATTCTGAGAGACATTAGTCAAACAAACAAAAG(+AAAAAA)GATCCCCATCACAATCTTACAGTACTGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606115352_cmr8 GCATTAATATCATTATTGCTGTGCCATATTTG(+AAAAAA)GATCCAAAGTTGCTCCAGGTTAATCCAACTT NGG 10 8 17 8 24 0 100 5 10
    240606115351_rgua GTATAAAGTTGGATTAACCTGGAGCAACTTTG(+AAAAAA)GATCCAAATATGGCACAGCAATAATGATATT NGG 10 8 17 8 24 0 100 5 10
    240606115350_e8k8 GGGCCATTCAGTCCTATTTCAATGGCAGTGTG(+AAAAAA)GATCCCTGCTCTTATCTAAGTATCTGGGTGT NGG 10 8 17 8 24 0 100 5 10