Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115302_mus0 AGGAGTCCGGCCGATCATGATCCTTCCTTGGG(+AAAAAA)GTACCAACCCTCAACTAGAGCCCCTGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606115301_r73f CTATGAAAACCTTCCCTTTGACAGTTCAGTTG(+AAAAAA)GATCCAGCTCCTGCTAGCCACTGAATTAATT NGG 10 8 17 8 24 0 100 5 10
    240606115300_u6ql ACACAAATTAATTCAGTGGCTAGCAGGAGCTG(+AAAAAA)GATCCAACTGAACTGTCAAAGGGAAGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606115258_i9pu TCTGTCTTCATAAAAGGGCAAGTTTGCTCACG(+AAAAAA)GTACCCGAGAGCAGGCACTTAGACCTGAATA NGG 10 8 17 8 24 0 100 5 10
    240606115257_vgpl CGCCATATTCAGGTCTAAGTGCCTGCTCTCGG(+AAAAAA)GTACCGTGAGCAAACTTGCCCTTTTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240606115255_57wk CCAGCTACTGCATCCAGTGGCCTGGGGCTCAG(+AAAAAA)GATCCATTTTCTACATTCATAAGAATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606115254_jf9q GGGCATGGGCATTCTTATGAATGTAGAAAATG(+AAAAAA)GATCCTGAGCCCCAGGCCACTGGATGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240606115252_er88 TATAGGACTTGATGAAATTAAGCAAGGTCCTG(+AAAAAA)GATCCCAGAAACAGGTCCACAGGTTCTTCGG NGG 10 8 17 8 24 0 100 5 10
    240606115250_t8iy GCATACCGAAGAACCTGTGGACCTGTTTCTGG(+AAAAAA)GATCCAGGACCTTGCTTAATTTCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606115249_wss8 CGATGGCTCTATTAATACACACACGGTGACAG(+AAAAAA)GTACCCTATTTAGACTAAGGCTACTAGAACA NGG 10 8 17 8 24 0 100 5 10