Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115231_xoeh CTTGGCGTTCAATAGCAAAATAAAGCTCCACG(+AAAAAA)GTACCAGGGGAATGAAATTTGGTTAAAACTG NGG 10 8 17 8 24 0 100 5 10
    240606115230_xkei GCTAGGAATACAGTGATGGAAAAATAAACAAG(+AAAAAA)GTACCTACTTCTTTGGAGACTGGAGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606115229_6w9r GGTTCCAACCACTCCAGTCTCCAAAGAAGTAG(+AAAAAA)GTACCTTGTTTATTTTTCCATCACTGTATTC NGG 10 8 17 8 24 0 100 5 10
    240606115226_l9zl TTCCTGACTTCCAGCCTTGCCTGACTCACCGG(+AAAAAA)GATCCTTATGTTTGGGCCTTCCCCATCCTAT NGG 10 8 17 8 24 0 100 5 10
    240606115225_nrs1 GTCCAATAGGATGGGGAAGGCCCAAACATAAG(+AAAAAA)GATCCCGGTGAGTCAGGCAAGGCTGGAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606115223_r7nb CTGAGATTACAGAAGTACCCAGAAGTGTCTGG(+AAAAAA)GATCCCTGTCACATCTGTGTCATGTTGTTAT NGG 10 8 17 8 24 0 100 5 10
    240606115221_jkyi TTCAAATAACAACATGACACAGATGTGACAGG(+AAAAAA)GATCCCAGACACTTCTGGGTACTTCTGTAAT NGG 10 8 17 8 24 0 100 5 10
    240606115220_x4cc TGATAGCCAACCTTGGTTTAGGCATCTCTCAG(+AAAAAA)GTACCTACAATTTCCCAAGTTCCAATAAATT NGG 10 8 17 8 24 0 100 5 10
    240606115219_vvkx AACAGAATTTATTGGAACTTGGGAAATTGTAG(+AAAAAA)GTACCTGAGAGATGCCTAAACCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606115217_qkk8 GACAAAATAGCCCATGGAAGCACAGTGAAGGG(+AAAAAA)GTACCCTGACAGACAGATGAGCCACAGGCCC NGG 10 8 17 8 24 0 100 5 10