Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115126_ntdx AGTGGCGAATGACACAAGCAAGCACCCTCATG(+AAAAAA)GATCCCCAATGAGAAAATTCCAGACTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606115125_4id4 AGGTCCTGCTTCTGCTCAGCAGCTTGAGGGAG(+AAAAAA)GTACCAGTGCCTCATTTTGTCTCTATCGGTT NGG 10 8 17 8 24 0 100 5 10
    240606115124_7noj CACAGAACCGATAGAGACAAAATGAGGCACTG(+AAAAAA)GTACCTCCCTCAAGCTGCTGAGCAGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606115121_8g5y CTCTGCCAGCCTGCTCTGTTCCTCTGTCCCTG(+AAAAAA)GATCCTCACTGGCAGCAGCTGTTACGCATTG NGG 10 8 17 8 24 0 100 5 10
    240606115120_lj9b TGCTCCAATGCGTAACAGCTGCTGCCAGTGAG(+AAAAAA)GATCCAGGGACAGAGGAACAGAGCAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606115118_kqn3 CTAGCCACACCTGTAGGGCAAGGTCACTGCAG(+AAAAAA)GTACCTCCCTGTCATTAGGCAGTGGACTTCG NGG 10 8 17 8 24 0 100 5 10
    240606115117_pzaz CTTCCCGAAGTCCACTGCCTAATGACAGGGAG(+AAAAAA)GTACCTGCAGTGACCTTGCCCTACAGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606115115_u47h ACCACATGGTGGCTCACAACCATCTGTAATGG(+AAAAAA)GATCCAATATCCTCTTTTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606115114_25qv GCTGTCTTCAGACACACCAAAAGAGGATATTG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606115112_xtbp CGCCTCCCTGGTGATGCCACCTAGGAGGGTTG(+AAAAAA)GATCCTGGACTCCAGCCCACCTTCTCTCTGG NGG 10 8 17 8 24 0 100 5 10