Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115055_y1j1 GGACTTGGTTGGTATAAGTACACTTGAGTTTG(+AAAAAA)GATCCCCAGCATTCCTATACAAATCTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240606115054_zibu CAAACAGTTTAATAGCAAATAAACAAAGGAAG(+AAAAAA)GTACCACACTTGGCAGATACAAAATGACTTT NGG 10 8 17 8 24 0 100 5 10
    240606115052_8cmq GACAAAAAGTCATTTTGTATCTGCCAAGTGTG(+AAAAAA)GTACCTTCCTTTGTTTATTTGCTATTAAACT NGG 10 8 17 8 24 0 100 5 10
    240606115050_5mt0 TGTACTCCCTGAGCAGAGCAGCCAGCCTCCTG(+AAAAAA)GATCCCCCACTGAGACCTGGAGGCCACCACA NGG 10 8 17 8 24 0 100 5 10
    240606115048_1djb CTCTTTGTGGTGGCCTCCAGGTCTCAGTGGGG(+AAAAAA)GATCCAGGAGGCTGGCTGCTCTGCTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606115046_l7j5 TCCCTGCATGTATGCCTGCAGGTCAGAAGAGG(+AAAAAA)GTACCAGATCCCGTTACAGATGGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606115045_1918 TGGTGGCTCACAACCATCTGTAACGGGATCTG(+AAAAAA)GTACCCTCTTCTGACCTGCAGGCATACATGC NGG 10 8 17 8 24 0 100 5 10
    240606115043_a5wt AGTAGGCTAGTCTGGCTGGCCAGCACCACAGG(+AAAAAA)GATCCTGTCTCTACCCTCCAGCATGGGCATC NGG 10 8 17 8 24 0 100 5 10
    240606115042_f8af CTTGTGATGCCCATGCTGGAGGGTAGAGACAG(+AAAAAA)GATCCCTGTGGTGCTGGCCAGCCAGACTAGC NGG 10 8 17 8 24 0 100 5 10
    240606115041_e5bd CCTGGCAGACCCTAAGGGTTTTAGGCAGCTGG(+AAAAAA)GATCCAGTGGGGAGGGCTGGCAAAGGAAAGG NGG 10 8 17 8 24 0 100 5 10