Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114954_9azy GGTTTTATTTAAGAGATCATTTTCCTTAAATG(+AAAAAA)GATCCATTATAATGCAGTTTACAAAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240606114953_sdyl GCCTTCAAGATATATCCAAACTAGGTACAAAG(+AAAAAA)GATCCACAAATATCAACAATTTGTAACCAAA NGG 10 8 17 8 24 0 100 5 10
    240606114952_p0i2 AGAATTTTGGTTACAAATTGTTGATATTTGTG(+AAAAAA)GATCCTTTGTACCTAGTTTGGATATATCTTG NGG 10 8 17 8 24 0 100 5 10
    240606114951_wrnb CAAGTTACACACTGGAAACAAAGCAGAACATG(+AAAAAA)GTACCTGCTTTTACTAACTCAAAACAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240606114949_2uol TTTGCTAACTTGTTTTGAGTTAGTAAAAGCAG(+AAAAAA)GTACCATGTTCTGCTTTGTTTCCAGTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606114947_djce TCAATCATTTAATTGACCGCAGTAGTCGCCGG(+AAAAAA)GTACCCGGCACTAAGCATGCACTACATCACA NGG 10 8 17 8 24 0 100 5 10
    240606114945_qg3c GCTTCTGTGATGTAGTGCATGCTTAGTGCCGG(+AAAAAA)GTACCCGGCGACTACTGCGGTCAATTAAATG NGG 10 8 17 8 24 0 100 5 10
    240606114943_7k25 TGAGCCACAAAGTTCCCACGAGTTATCATTAG(+AAAAAA)GATCCTGGCCACTGTTTTTGCCATGGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606114942_zvzd GGAAGGAAAACCATGGCAAAAACAGTGGCCAG(+AAAAAA)GATCCTAATGATAACTCGTGGGAACTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606114940_qstk AAATCACTGACAATGCCTATATTAGTTAATGG(+AAAAAA)GATCCATCTCAACCCTAAAAAGAAGGAAAAA NGG 10 8 17 8 24 0 100 5 10