Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114939_9l2z ATTCATTTTTCCTTCTTTTTAGGGTTGAGATG(+AAAAAA)GATCCCATTAACTAATATAGGCATTGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606114937_u6a1 TTGCCTGAACTGATATCTTGGTTTTTCATCCG(+AAAAAA)GATCCAGTCAGGACAGAGACATCAGAAACTA NGG 10 8 17 8 24 0 100 5 10
    240606114936_02p0 TGTATTAGTTTCTGATGTCTCTGTCCTGACTG(+AAAAAA)GATCCGGATGAAAAACCAAGATATCAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606114934_0aic AAACCCAAAGCATACGCAGGAGTCAGTTCCTG(+AAAAAA)GTACCGTAGAGTGAGAAAGACACCTGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606114933_m8eq CTTCCCTGCACAGGTGTCTTTCTCACTCTACG(+AAAAAA)GTACCAGGAACTGACTCCTGCGTATGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606114931_gc0q CAGGGTAGGTGTAGTTCTGAGAGTTGTGAGAG(+AAAAAA)GATCCAAAGATCACTTGGGCAGGTTTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240606114930_0gbr AAATCTACTCAAACCTGCCCAAGTGATCTTTG(+AAAAAA)GATCCTCTCACAACTCTCAGAACTACACCTA NGG 10 8 17 8 24 0 100 5 10
    240606114929_cxb1 TGAAGACAGCCCATTTCACAGACAATGACATG(+AAAAAA)GTACCCAGCAGCATCTCCCCTTCTGCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606114927_lbxp GTCCTTACAAGCAGAAGGGGAGATGCTGCTGG(+AAAAAA)GTACCATGTCATTGTCTGTGAAATGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606114926_gqyg AGGGATGTGTTCTATGGCCTGTGTGCTTTGTG(+AAAAAA)GTACCACCCAGCCCAGGCAGAAACTGGGCTT NGG 10 8 17 8 24 0 100 5 10