Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114853_97q5 AAGGAAAAAACAAATTTTAAACTTAATTAGAG(+AAAAAA)GATCCAAATGATCTCTTAACATGGTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240606114852_hmvf GATGTTTACATTTACCATGTTCCCCTTATTAG(+AAAAAA)GATCCGCACAGCCAAGTTCCGGGCCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606114851_z645 CCTGAGGAAGAGGCCCGGAACTTGGCTGTGCG(+AAAAAA)GATCCTAATAAGGGGAACATGGTAAATGTAA NGG 10 8 17 8 24 0 100 5 10
    240606114850_7q0k CCACACACACACACACACGGGCCAGTCAGACG(+AAAAAA)GATCCAGGTGTCCAGTTGTGGCTGATCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606114847_9y1f CCCGAGGCTGATCAGCCACAACTGGACACCTG(+AAAAAA)GATCCGTCTGACTGGCCCGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606114846_8w04 TTAGGGCCCAAGAGACGGCCCAGCAGGTAAAG(+AAAAAA)GTACCTGCTGCCAGGTCTAATGAGCTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606114845_0bzw AATCAAACTTAGCTCATTAGACCTGGCAGCAG(+AAAAAA)GTACCTTTACCTGCTGGGCCGTCTCTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606114843_cvjk ACATGTGGTTGCCATGGCTTAATGTATGGACG(+AAAAAA)GATCCTCCACTGTCTTGCAATTCATTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606114841_v0ib TTTTTCAACAAATGAATTGCAAGACAGTGGAG(+AAAAAA)GATCCGTCCATACATTAAGCCATGGCAACCA NGG 10 8 17 8 24 0 100 5 10
    240606114840_bad1 CACCTTTTCTACAGCAATTTTTAGTAATTATG(+AAAAAA)GTACCAAGTTCCCACAAACCTTTGGGTGACT NGG 10 8 17 8 24 0 100 5 10