Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114909_slac GGACACTGTCAATGTTCATAGCTTAAACCTGG(+AAAAAA)GTACCTGGTCAAAATGCTTAGGAAACATTAA NGG 10 8 17 8 24 0 100 5 10
    240606114907_oe5w CCTTGAGACCTTTATAAGCAAAAGCAAACCAG(+AAAAAA)GTACCCACCAGTTTGCTCAGACCCAGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606114905_fkq0 ACAAAGTCTGCTGGGTCTGAGCAAACTGGTGG(+AAAAAA)GTACCTGGTTTGCTTTTGCTTATAAAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606114903_5prc AAAGTTAAAAATTCCTTAATTTTTTATTCCTG(+AAAAAA)GTACCACTACCACAATTTACAGGGCAATATA NGG 10 8 17 8 24 0 100 5 10
    240606114902_7daj TCAGGTATATTGCCCTGTAAATTGTGGTAGTG(+AAAAAA)GTACCAGGAATAAAAAATTAAGGAATTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606114900_98ux CACTCAGGCCTGGAACTCGGTGCCCCCTCTCG(+AAAAAA)GATCCTGGCCTCACATGGGCCTCAGACCTAA NGG 10 8 17 8 24 0 100 5 10
    240606114858_szqc TTTTCTTAGGTCTGAGGCCCATGTGAGGCCAG(+AAAAAA)GATCCGAGAGGGGGCACCGAGTTCCAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606114857_dnic ACTCTAGGATTGGAAAAAAAAAAAAAAAAAAG(+AAAAAA)GTACCGCAGCCTCTTGGCAAATAAGGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606114856_2f95 GTTCCAGAAACCTTATTTGCCAAGAGGCTGCG(+AAAAAA)GTACCTTTTTTTTTTTTTTTTTTCCAATCCT NGG 10 8 17 8 24 0 100 5 10
    240606114855_z9ix AGCTCCTTGTCACCATGTTAAGAGATCATTTG(+AAAAAA)GATCCTCTAATTAAGTTTAAAATTTGTTTTT NGG 10 8 17 8 24 0 100 5 10