Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114809_44m8 AGCACTGGGCTCGCTTGGCATGTCCTGGGTTG(+AAAAAA)GATCCCAGTACTCCAACTGAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606114807_01ri CTTTTTTTTTTTTTTTTCAGTTGGAGTACTGG(+AAAAAA)GATCCAACCCAGGACATGCCAAGCGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606114806_41ek GCAAGCTGCTTCCCCCGAGCCTTCAGCACAGG(+AAAAAA)GATCCTCTCAACCACAGGCAGCCGGGTTGCC NGG 10 8 17 8 24 0 100 5 10
    240606114805_oysm TTGCTGGCAACCCGGCTGCCTGTGGTTGAGAG(+AAAAAA)GATCCCTGTGCTGAAGGCTCGGGGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606114803_3ye4 AAGGCATAGCAGTGGTACAGGGAACCCAGTGG(+AAAAAA)GTACCCCGCCATCTCTCTTCCAAAGATGAAC NGG 10 8 17 8 24 0 100 5 10
    240606114801_vxba GGGAGGTTCATCTTTGGAAGAGAGATGGCGGG(+AAAAAA)GTACCCACTGGGTTCCCTGTACCACTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240606114759_nnoj TGTGTCAGCTACTGACAGAGCTGTGCTCTAGG(+AAAAAA)GTACCAGAGGGGCACAAGCATGGCCGATGAT NGG 10 8 17 8 24 0 100 5 10
    240606114757_15a4 CCAGAATCATCGGCCATGCTTGTGCCCCTCTG(+AAAAAA)GTACCCTAGAGCACAGCTCTGTCAGTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606114756_2ggs AATTATTCAATCACCTGGAAAAAAAAGTCTTG(+AAAAAA)GTACCAATAAAATATGTTTTTCTGTCATCTT NGG 10 8 17 8 24 0 100 5 10
    240606114755_iqzi GAAAGAAGATGACAGAAAAACATATTTTATTG(+AAAAAA)GTACCAAGACTTTTTTTTCCAGGTGATTGAA NGG 10 8 17 8 24 0 100 5 10