Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114753_1f0n TAAAATCAAATGAAGGTTTAAGATCACATAAG(+AAAAAA)GATCCTGCTTCTGTTAGTTAAGGAAACTGCT NGG 10 8 17 8 24 0 100 5 10
    240606114752_0vi9 CTTAGAGCAGTTTCCTTAACTAACAGAAGCAG(+AAAAAA)GATCCTTATGTGATCTTAAACCTTCATTTGA NGG 10 8 17 8 24 0 100 5 10
    240606114751_tmrw TGATGCAGCTGACTGATGAAGGTACTGGTGAG(+AAAAAA)GTACCTCTAGGGCTAGGACCAGATGCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240606114750_0vby TGAAGTCACTGCATCTGGTCCTAGCCCTAGAG(+AAAAAA)GTACCTCACCAGTACCTTCATCAGTCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606114748_1ixo GTCTTCTTCTCTGCCAGATAACAGAGCCATTG(+AAAAAA)GATCCACTTATTAAAGACTTCTTATCACTTA NGG 10 8 17 8 24 0 100 5 10
    240606114746_cixf AATGTTAAGTGATAAGAAGTCTTTAATAAGTG(+AAAAAA)GATCCAATGGCTCTGTTATCTGGCAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606114745_3898 CTTTTTCTAGAGTCCTTTCTACACACACTTTG(+AAAAAA)GTACCCTGGGGAGTTTGGATGCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606114742_e3yu CCTCCTTCCTGGTTGCATCCAAACTCCCCAGG(+AAAAAA)GTACCAAAGTGTGTGTAGAAAGGACTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606114740_t78c CAAGACCTTCAAGAAGGTGGGCTCCGGGGATG(+AAAAAA)GTACCATTGCTCTTTCCTAGCTAACCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606114739_qlv2 CCGTATCTAGGGTTAGCTAGGAAAGAGCAATG(+AAAAAA)GTACCATCCCCGGAGCCCACCTTCTTGAAGG NGG 10 8 17 8 24 0 100 5 10