Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114706_lzx0 CTAAATTTAAAAGTCTTAATGAATCTCCTTTG(+AAAAAA)GATCCCTGAATGGTCCAAGATCTTGGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606114705_gmq0 GCTCCAGACTCCAAGATCTTGGACCATTCAGG(+AAAAAA)GATCCAAAGGAGATTCATTAAGACTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606114703_p1zk AAAGTATGAATGTGTTCTGCACAGCAAACTGG(+AAAAAA)GATCCTTATCAGTACAACCTGAAGACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240606114702_nl37 AGGGACTCTAGTCTTCAGGTTGTACTGATAAG(+AAAAAA)GATCCCAGTTTGCTGTGCAGAACACATTCAT NGG 10 8 17 8 24 0 100 5 10
    240606114659_2ri2 AGAATTATCAAGGAACACCTAAAAATGACCAG(+AAAAAA)GATCCAAATAGGTTATTTATATTATATTTCT NGG 10 8 17 8 24 0 100 5 10
    240606114658_j7rf TATAGAGAAATATAATATAAATAACCTATTTG(+AAAAAA)GATCCTGGTCATTTTTAGGTGTTCCTTGATA NGG 10 8 17 8 24 0 100 5 10
    240606114657_4eqp AGCTGAGTTTTGCATAATGATTTTGTATCTTG(+AAAAAA)GTACCTCACTGAATTCATTTATGATTTATGA NGG 10 8 17 8 24 0 100 5 10
    240606114656_2lto ATCCATCATAAATCATAAATGAATTCAGTGAG(+AAAAAA)GTACCAAGATACAAAATCATTATGCAAAACT NGG 10 8 17 8 24 0 100 5 10
    240606114655_4c5g TATAATAAAATAATACTAAAAGAGAGCATGTG(+AAAAAA)GATCCATACACCAGATTAATGCGGGGATAGG NGG 10 8 17 8 24 0 100 5 10
    240606114653_2b8p CATACCCTATCCCCGCATTAATCTGGTGTATG(+AAAAAA)GATCCACATGCTCTCTTTTAGTATTATTTTA NGG 10 8 17 8 24 0 100 5 10