Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114609_a513 GTAACCCCCTCCTTCCAACTTCTTAGGAGAAG(+AAAAAA)GATCCAAGCACGTATCTGTGCACACAAATTC NGG 10 8 17 8 24 0 100 5 10
    240606114607_kxzk GTGTAGAATTTGTGTGCACAGATACGTGCTTG(+AAAAAA)GATCCTTCTCCTAAGAAGTTGGAAGGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606114606_od0v TGGTTCAAGAGGTGGGCTGCACCTGTCTGGAG(+AAAAAA)GATCCCTGCATGCCATCAATAAGACTCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606114605_m2qd TGTGACACTGAGTCTTATTGATGGCATGCAGG(+AAAAAA)GATCCTCCAGACAGGTGCAGCCCACCTCTTG NGG 10 8 17 8 24 0 100 5 10
    240606114603_4oj0 AATTCATAGTTATTTATTTTATTCACATTAGG(+AAAAAA)GATCCTGAGGAAGTAGAATCTATATAGGACA NGG 10 8 17 8 24 0 100 5 10
    240606114601_ehtk ATACCTGTCCTATATAGATTCTACTTCCTCAG(+AAAAAA)GATCCCTAATGTGAATAAAATAAATAACTAT NGG 10 8 17 8 24 0 100 5 10
    240606114559_in5r ACCCCAGAGGTAGTATGGCAAAAACGAGCATG(+AAAAAA)GATCCTATCAGGTCACAACCGACAAAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240606114558_idxm ATTTAATTACTTTGTCGGTTGTGACCTGATAG(+AAAAAA)GATCCATGCTCGTTTTTGCCATACTACCTCT NGG 10 8 17 8 24 0 100 5 10
    240606114557_osai GGCAAAATAAATAGTTAAAACAAGTGATGCAG(+AAAAAA)GATCCCATTTCATGCTCATGATCCCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240606114555_ukuc AATTCTTTAATGGGATCATGAGCATGAAATGG(+AAAAAA)GATCCTGCATCACTTGTTTTAACTATTTATT NGG 10 8 17 8 24 0 100 5 10