Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114527_cu0q AGTCCTCTAGTCTTGGCTGTGGGGGCTTCCAG(+AAAAAA)GTACCACTTGGGGAGAGGACTCAAGTAAGAC NGG 10 8 17 8 24 0 100 5 10
    240606114526_2ulb CACTTGTCTTACTTGAGTCCTCTCCCCAAGTG(+AAAAAA)GTACCTGGAAGCCCCCACAGCCAAGACTAGA NGG 10 8 17 8 24 0 100 5 10
    240606114525_3bvp GGGTTGGGAACAAGGGGGTGGGGTCATCAGAG(+AAAAAA)GTACCTGTTACTTTAGGGACCCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606114523_w3f6 CTCTCAGACAGAGAGGGTCCCTAAAGTAACAG(+AAAAAA)GTACCTCTGATGACCCCACCCCCTTGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606114522_4i13 CAGACTGGAGTTTTCTGCCTGTTTGTCTCTCG(+AAAAAA)GATCCTCAGAATAGCCGTGTGATGTGAGGTA NGG 10 8 17 8 24 0 100 5 10
    240606114521_q2b1 CTGCCTACCTCACATCACACGGCTATTCTGAG(+AAAAAA)GATCCGAGAGACAAACAGGCAGAAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240606114520_g0cm CTCATGACTTTATATATTCAAAGCTTTTAATG(+AAAAAA)GTACCTGTTACACAGCAAGTACTCTGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240606114519_nzlc TCAGCATTCACAGAGTACTTGCTGTGTAACAG(+AAAAAA)GTACCATTAAAAGCTTTGAATATATAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606114516_ruww TAACCAATTGCTTAGGGGCAGGAAGATGTGGG(+AAAAAA)GATCCCACCTTGCCAGAGCTCTTACCATCTT NGG 10 8 17 8 24 0 100 5 10
    240606114515_zyzg TGGCAAAGATGGTAAGAGCTCTGGCAAGGTGG(+AAAAAA)GATCCCCACATCTTCCTGCCCCTAAGCAATT NGG 10 8 17 8 24 0 100 5 10