Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114541_smk7 TAATAACAGACCAGCGATTTTATAGAGGCCAG(+AAAAAA)GATCCTGAACTGGGGCATCCTCTCTGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606114540_cuze GCCTGCACTCCAGAGAGGATGCCCCAGTTCAG(+AAAAAA)GATCCTGGCCTCTATAAAATCGCTGGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606114538_x4kg TTGGACTTTGTAAGCCTGAAAAAGAATATTTG(+AAAAAA)GATCCCTCTACTAAACAAGGGCTATGTATTA NGG 10 8 17 8 24 0 100 5 10
    240606114537_6sgp CTGTATAATACATAGCCCTTGTTTAGTAGAGG(+AAAAAA)GATCCAAATATTCTTTTTCAGGCTTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240606114535_ga2y TAAGGATAAATGCATTAAGTATAACTAATTTG(+AAAAAA)GTACCATACAAGTATTTTCTTTGGAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240606114534_7bbx ACATACAAAATTCCAAAGAAAATACTTGTATG(+AAAAAA)GTACCAAATTAGTTATACTTAATGCATTTAT NGG 10 8 17 8 24 0 100 5 10
    240606114533_p0s9 ATGCCCCTTTGTAATTTTTCTTGCCTTCTGTG(+AAAAAA)GTACCTGCTCTCTAAAGCAGTAGTTTCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606114531_02am AAAGCTGGAGAAACTACTGCTTTAGAGAGCAG(+AAAAAA)GTACCACAGAAGGCAAGAAAAATTACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606114530_6c4l TGTCAGTTTTTCTCTTTAGTGAAATGATACAG(+AAAAAA)GTACCAGCAAAGTGACAATATGTCCTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240606114529_0toy TGGACATCAAAGGACATATTGTCACTTTGCTG(+AAAAAA)GTACCTGTATCATTTCACTAAAGAGAAAAAC NGG 10 8 17 8 24 0 100 5 10