Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115024_2fil GCTTCTTTAGCTTCTCACTGATCACCTGTTAG(+AAAAAA)GATCCTGTGAAAACAGGCCTGGCTGACCTGG NGG 10 8 17 8 24 0 100 5 10
    240606115023_n31u TAGAAGAAGATGGAGACACTGTGTCCTAAGAG(+AAAAAA)GATCCTGTACCCCAGGCTAGGGTAATTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606115022_uca2 CTGGGGGAGAATTACCCTAGCCTGGGGTACAG(+AAAAAA)GATCCTCTTAGGACACAGTGTCTCCATCTTC NGG 10 8 17 8 24 0 100 5 10
    240606115020_f7yu GTCCAAAATCTTTCTGGCTAGCCAGTCAGTTG(+AAAAAA)GATCCCAAGTCCTTTGGGGTAGCATGACTCT NGG 10 8 17 8 24 0 100 5 10
    240606115019_tfx2 GTCACAGAGTCATGCTACCCCAAAGGACTTGG(+AAAAAA)GATCCAACTGACTGGCTAGCCAGAAAGATTT NGG 10 8 17 8 24 0 100 5 10
    240606115017_1tyw CCATCTTCGCCTCTTGTTTGCGACCCGGCCTG(+AAAAAA)GTACCTTTCCTAGGCCTCCTCGCTCTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606115015_2w64 GGGAAGAAAAAGAGCGAGGAGGCCTAGGAAAG(+AAAAAA)GTACCAGGCCGGGTCGCAAACAAGAGGCGAA NGG 10 8 17 8 24 0 100 5 10
    240606115014_vv2m CTTTCAATCTCAATCACTCAACAGTAAATTTG(+AAAAAA)GTACCTTCTGTAGGCTCTGCCTTCTGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606115013_bxc9 GGACTCTGCTCAGAAGGCAGAGCCTACAGAAG(+AAAAAA)GTACCAAATTTACTGTTGAGTGATTGAGATT NGG 10 8 17 8 24 0 100 5 10
    240606115011_9fqq AAAATCTGAATATTTTTTTAAGATCACCTAAG(+AAAAAA)GTACCATTTTAAAGATTCCAAGACCCTAGAA NGG 10 8 17 8 24 0 100 5 10