Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115110_fy6k CTAGGCCAGAGAGAAGGTGGGCTGGAGTCCAG(+AAAAAA)GATCCAACCCTCCTAGGTGGCATCACCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606115109_2r7z CCCGAGAGCCACAGGCTGTGTCCATGAACTTG(+AAAAAA)GATCCCTGAGCACTTGAGAATGAACTCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606115107_ptwy GAACCTCTTGAGTTCATTCTCAAGTGCTCAGG(+AAAAAA)GATCCAAGTTCATGGACACAGCCTGTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606115106_opkx GCCTCCGGGCTAATCCCAAATAAAACCTTTGG(+AAAAAA)GTACCAAGCAAAAGTTCTAGCTCTTGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606115104_o3kb GGTAGCTCACCAAGAGCTAGAACTTTTGCTTG(+AAAAAA)GTACCCAAAGGTTTTATTTGGGATTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240606115103_yme3 GCCTCCGGGCTAATCCCAAATAAAACCTTTGG(+AAAAAA)GTACCAAGCAAAAGTTCTAGCTCTTGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606115102_nkjl GGTAGCTCACCAAGAGCTAGAACTTTTGCTTG(+AAAAAA)GTACCCAAAGGTTTTATTTGGGATTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240606115100_yzyg ACCCCAGGAACTCCTAAACAGTTTAGGAGCTG(+AAAAAA)GATCCTCCCACTCTGGGATGACCCAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240606115059_lvst TCGGGAACATTTGGGTCATCCCAGAGTGGGAG(+AAAAAA)GATCCAGCTCCTAAACTGTTTAGGAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606115056_ttcx CATCAGGACAAGATTTGTATAGGAATGCTGGG(+AAAAAA)GATCCAAACTCAAGTGTACTTATACCAACCA NGG 10 8 17 8 24 0 100 5 10