Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115158_y5bi TACTTGGCAATTCACACACATCATCCATGCGG(+AAAAAA)GTACCAGCAGTGGTGGGGCTCTGTTCTGACG NGG 10 8 17 8 24 0 100 5 10
    240606115156_7shp AAAGTGTACATCAAGACCAACAACATCCTGTG(+AAAAAA)GTACCGCAGGATCCAGGAAAGCAGCCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606115154_y4yz TGTCCACACAGGCTGCTTTCCTGGATCCTGCG(+AAAAAA)GTACCACAGGATGTTGTTGGTCTTGATGTAC NGG 10 8 17 8 24 0 100 5 10
    240606115152_alox ATCAAGACCAACAACATCCTGTGGTACCGCAG(+AAAAAA)GATCCAGGAAAGCAGCCTGTGTGGACACACA NGG 10 8 17 8 24 0 100 5 10
    240606115151_finj AATGCTGTGTGTCCACACAGGCTGCTTTCCTG(+AAAAAA)GATCCTGCGGTACCACAGGATGTTGTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606115149_0vml TTGACTGAGTAAAGGAGAGGTGGTTTCTATGG(+AAAAAA)GATCCTTTCTGTCTGCTGGGGGACATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606115148_a0us CCAATGCCCCATGTCCCCCAGCAGACAGAAAG(+AAAAAA)GATCCCATAGAAACCACCTCTCCTTTACTCA NGG 10 8 17 8 24 0 100 5 10
    240606115146_v0nb AGACTGAGGCTTGTGGCCATTGTGCCAAATGG(+AAAAAA)GTACCTTGCTGAAACACAGGGTTACAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606115145_2yd4 GGGGGCACACTGTAACCCTGTGTTTCAGCAAG(+AAAAAA)GTACCCATTTGGCACAATGGCCACAAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606115143_pfnr AGCTCAATGTGAGCAGGGGCTGCCGACTGAGG(+AAAAAA)GTACCTGGCACATATCTTTTTCCATAACACA NGG 10 8 17 8 24 0 100 5 10