Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115447_uvyn CAGAAATCCGGATGAGGGGTATCGGCAAACAG(+AAAAAA)GATCCAGGTGGTTGCAAAATCTAGCGAACAG NGG 10 8 17 8 24 0 100 5 10
    240606115446_cfa9 CTGTGTCTCATATTTATATCATAAAAACATTG(+AAAAAA)GATCCCTATAACTTCAAAGTCGTGTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606115444_vji1 AGTGAAGAAGCACACGACTTTGAAGTTATAGG(+AAAAAA)GATCCAATGTTTTTATGATATAAATATGAGA NGG 10 8 17 8 24 0 100 5 10
    240606115443_c2jz AGGTTTCTAATTGCCCAGCTGCTAGGTAACTG(+AAAAAA)GTACCTTTTAAAGCCCCCTGTAATTTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606115442_pvjf GAGGGAAGGAAAATTACAGGGGGCTTTAAAAG(+AAAAAA)GTACCAGTTACCTAGCAGCTGGGCAATTAGA NGG 10 8 17 8 24 0 100 5 10
    240606115440_mwyf ATGAAAATGCCAAAATTAATCCCTTTTAGTTG(+AAAAAA)GATCCTATTCTATGTTGACCTTTTGTAATCA NGG 10 8 17 8 24 0 100 5 10
    240606115439_z8ai GTTATTGATTACAAAAGGTCAACATAGAATAG(+AAAAAA)GATCCAACTAAAAGGGATTAATTTTGGCATT NGG 10 8 17 8 24 0 100 5 10
    240606115438_dhe0 AGACCAAGTTTCTCACTAACCAGGTACAAAAG(+AAAAAA)GATCCAACCAACGTATGAGTTACGGTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240606115436_um5m AGAAACAAACACCGTAACTCATACGTTGGTTG(+AAAAAA)GATCCTTTTGTACCTGGTTAGTGAGAAACTT NGG 10 8 17 8 24 0 100 5 10
    240606115435_3ztj TTCTATTAAAACCTGTCCCTGGTACTATCATG(+AAAAAA)GTACCAAGCTGTGTAAAACACTGCACTGAAT NGG 10 8 17 8 24 0 100 5 10