Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115518_p79f AGTTCAGTGACGCTAAGGTACTCCATCTTTGG(+AAAAAA)GATCCAGTTGGAGTATCTCACTGACTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606115517_z8oe TGGTGGGAGTTACGGCTGCTGGTGGGAGTCTG(+AAAAAA)GTACCTTTCTGGTTGAGCAATGCAGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606115516_qfp3 GTGGAATTTTGCTGCATTGCTCAACCAGAAAG(+AAAAAA)GTACCAGACTCCCACCAGCAGCCGTAACTCC NGG 10 8 17 8 24 0 100 5 10
    240606115514_dvl2 GATGCAGGGCTGGAAGGAGCCAAGCAAGGAGG(+AAAAAA)GTACCCAGGAGGAGCTGGCAGGGCGAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606115512_6wip CCAGGTGTTCTCGCCCTGCCAGCTCCTCCTGG(+AAAAAA)GTACCCTCCTTGCTTGGCTCCTTCCAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606115510_rfpp CTCTACTCTTTCTTGCCATCGCCCCTGGCATG(+AAAAAA)GATCCAGAGACTCAGGCTACAACAGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606115509_w6nz AAAGTGGCTTCCTGTTGTAGCCTGAGTCTCTG(+AAAAAA)GATCCATGCCAGGGGCGATGGCAAGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606115508_yb8a AGCCAAGCTGCGTGAGTGGAGTGTGGGGAATG(+AAAAAA)GATCCCTTGGGGGCTACAGAAAGAGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606115506_biza AAGAGCCTTCACTCTTTCTGTAGCCCCCAAGG(+AAAAAA)GATCCATTCCCCACACTCCACTCACGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606115504_pqf3 ACACACACAGAGACCTGCCCCTGCTGTGGTTG(+AAAAAA)GATCCAATAAACTTTCTGGAGGCATAGCTTG NGG 10 8 17 8 24 0 100 5 10