Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115532_i9h8 TGCATTTATTTAAAATGTGTATATTTTAATAG(+AAAAAA)GATCCTTGTAAGGGCTAACTTTTAATAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606115531_w9fp TCTAATCTCTGTCAAAAGGGAGGTTAGGAAAG(+AAAAAA)GTACCCCCTCCCTTGTAAACACTGAAATACA NGG 10 8 17 8 24 0 100 5 10
    240606115529_56rp AGATCTGTATTTCAGTGTTTACAAGGGAGGGG(+AAAAAA)GTACCTTTCCTAACCTCCCTTTTGACAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606115527_qcz8 CTGCCAGAGGCTTGCCCAAGAAAAGGAGTCGG(+AAAAAA)GTACCTGTCAGATGCTACCTGGAGGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606115526_5qpt GATGACCTGACCCTCCAGGTAGCATCTGACAG(+AAAAAA)GTACCCGACTCCTTTTCTTGGGCAAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606115525_mjzx AGGTTGGGATGTCCCTGTTGCTAGCCCATTAG(+AAAAAA)GATCCTAGCTCCAATTCTGACCTTGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606115523_ha9x CTTGACTTTTTCAAGGTCAGAATTGGAGCTAG(+AAAAAA)GATCCTAATGGGCTAGCAACAGGGACATCCC NGG 10 8 17 8 24 0 100 5 10
    240606115522_970y CTCCATGATGGTGGGACACCTGCTCCCGTGTG(+AAAAAA)GATCCTATAGCTAGTGTCCTTCAGTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240606115521_zy0w GCACGCAGTGGACTGAAGGACACTAGCTATAG(+AAAAAA)GATCCACACGGGAGCAGGTGTCCCACCATCA NGG 10 8 17 8 24 0 100 5 10
    240606115520_mju0 GGGTTTTTCTAGTCAGTGAGATACTCCAACTG(+AAAAAA)GATCCCAAAGATGGAGTACCTTAGCGTCACT NGG 10 8 17 8 24 0 100 5 10