Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115549_dzts ACTTTTCAGCCAGTATCCAGGTCAGGAATGTG(+AAAAAA)GATCCTGGCAGTGCTGAGGCTTCAGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240606115547_bwua TGTAGCCTTGCCCTGTCTTGGGCCTGGTGGAG(+AAAAAA)GATCCTGGAGCCTCTTGGTGGTGCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240606115545_1757 AGAAAGGACAATGCACCACCAAGAGGCTCCAG(+AAAAAA)GATCCTCCACCAGGCCCAAGACAGGGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240606115542_lck8 ATTTGGCAACCTAACCAGGCAGCTGCCCCTGG(+AAAAAA)GATCCCTAGCTTCTCCAACCTGAGCACCAGC NGG 10 8 17 8 24 0 100 5 10
    240606115541_v115 GGAATGCTGGTGCTCAGGTTGGAGAAGCTAGG(+AAAAAA)GATCCCAGGGGCAGCTGCCTGGTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240606115540_4smx GAGCCCTTTATTGGCTTGGATTAGCTGTGTAG(+AAAAAA)GATCCAGGGACTGTCCTCGTCCTCGGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606115537_phw3 GCAAGCCAACCCGAGGACGAGGACAGTCCCTG(+AAAAAA)GATCCTACACAGCTAATCCAAGCCAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240606115536_aczn AATGTCTCCTCTCCAGTAGCTCCACAGAGGTG(+AAAAAA)GTACCTCAGCCAAAGCCATTCCTAACCCATG NGG 10 8 17 8 24 0 100 5 10
    240606115535_i94a AGGTTCATGGGTTAGGAATGGCTTTGGCTGAG(+AAAAAA)GTACCACCTCTGTGGAGCTACTGGAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606115534_ikzr AAAAAGCTTTATTAAAAGTTAGCCCTTACAAG(+AAAAAA)GATCCTATTAAAATATACACATTTTAAATAA NGG 10 8 17 8 24 0 100 5 10