Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114412_q28c GCACAGCACCTACCAGCCCTGGCCCTTCCTTG(+AAAAAA)GTACCATCTCCACCACTGCTGGACTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240606114411_qpnz GGGTAGGTGGGAGTCCAGCAGTGGTGGAGATG(+AAAAAA)GTACCAAGGAAGGGCCAGGGCTGGTAGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606114409_0qx2 ATATGATGAGCCCAAGAGATCTGGGATGAATG(+AAAAAA)GATCCCTAGATACGGTAGGCCCAGATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606114408_obqw GGTGGGGGAGATCTGGGCCTACCGTATCTAGG(+AAAAAA)GATCCATTCATCCCAGATCTCTTGGGCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606114406_ecea GCCACCCCACAGCTGGGGCTTGGAGGCTTCTG(+AAAAAA)GATCCAGGGGGTTGTGGCTTGCATTGCATCT NGG 10 8 17 8 24 0 100 5 10
    240606114403_ayof GGTACAGATGCAATGCAAGCCACAACCCCCTG(+AAAAAA)GATCCAGAAGCCTCCAAGCCCCAGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606114401_eofq GGGGAAACCCAGGGAAGTTATATAAACATTGG(+AAAAAA)GTACCACTGTGTCATTGCAGTTCTGTGGCCC NGG 10 8 17 8 24 0 100 5 10
    240606114400_o27l AGACAGGGCCACAGAACTGCAATGACACAGTG(+AAAAAA)GTACCCAATGTTTATATAACTTCCCTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606114358_1luj GCAGGGCAATGGCACGGCTGTGAGGTTGCGGG(+AAAAAA)GTACCCAGGAGTTTCTTAGCAAAGATGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606114356_h4fc GTCTAGAAGCATCTTTGCTAAGAAACTCCTGG(+AAAAAA)GTACCCCGCAACCTCACAGCCGTGCCATTGC NGG 10 8 17 8 24 0 100 5 10