Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114300_w33d GCCCGAGGTTCTGGTGGCCCAGCAACTGCAGG(+AAAAAA)GATCCATAAATCCAGAGGCCAGGATTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606114259_fhvs ACTGAGCCTGAATCCTGGCCTCTGGATTTATG(+AAAAAA)GATCCCTGCAGTTGCTGGGCCACCAGAACCT NGG 10 8 17 8 24 0 100 5 10
    240606114258_wsyw ACAAGGGGAAGGAAGCCCAGGTAGAGGGTATG(+AAAAAA)GATCCCTCGAACCTGTCAGCAGACGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606114256_zj4t ACCGCGTGTTCCGTCTGCTGACAGGTTCGAGG(+AAAAAA)GATCCATACCCTCTACCTGGGCTTCCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606114254_9k5u ATTCCTGGCCCTAGAAAGGAGGGTCTGCCGAG(+AAAAAA)GTACCAGGGGCCTGGCCTCACTGGCTGGACC NGG 10 8 17 8 24 0 100 5 10
    240606114251_owfi GGTGTGGTCCAGCCAGTGAGGCCAGGCCCCTG(+AAAAAA)GTACCTCGGCAGACCCTCCTTTCTAGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606114249_754m GCAGTGGGGGCTTTCTCAGGAGAGATGCCAGG(+AAAAAA)GATCCCCAGGGACTGCACACCCCACTATCTC NGG 10 8 17 8 24 0 100 5 10
    240606114246_cb34 TCTAGGAGATAGTGGGGTGTGCAGTCCCTGGG(+AAAAAA)GATCCCTGGCATCTCTCCTGAGAAAGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606114245_mg9j TACCTTCTATGAGCGCTGCCTACCTGTTTGTG(+AAAAAA)GTACCCTGTGATGGAGGGCCTCACAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240606114243_ckbw ACTAGTGTGGCTGTGAGGCCCTCCATCACAGG(+AAAAAA)GTACCACAAACAGGTAGGCAGCGCTCATAGA NGG 10 8 17 8 24 0 100 5 10