Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606114153_x7iy ACCCGCAGCTCCTAGTCTCTAATGCCTGGTAG(+AAAAAA)GATCCCATGGGCCTTTGCGACGCCACGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606114151_amdf GTTGCCTAACGTGGCGTCGCAAAGGCCCATGG(+AAAAAA)GATCCTACCAGGCATTAGAGACTAGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606114150_y2ug GGACATCAATCTAAAGCCTCGAGGCCATGTTG(+AAAAAA)GATCCACCCTGACCCGGTCCTGTAGCAACTA NGG 10 8 17 8 24 0 100 5 10
    240606114148_rmiw TTTTTTAGTTGCTACAGGACCGGGTCAGGGTG(+AAAAAA)GATCCAACATGGCCTCGAGGCTTTAGATTGA NGG 10 8 17 8 24 0 100 5 10
    240606114146_rwcv TTTAGCAAAAGAAATAATGTCTTAGTACTTGG(+AAAAAA)GTACCACGGCGAGAGACAGGTCACCTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606114144_dnqq GCAGGGTGGCAGGTGACCTGTCTCTCGCCGTG(+AAAAAA)GTACCCAAGTACTAAGACATTATTTCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240606114143_5jks CTCCTCAGAGGGAGATGACAGGAGTGGAGATG(+AAAAAA)GTACCGATAGTCGTTGCTAGTCTGTCTACCG NGG 10 8 17 8 24 0 100 5 10
    240606114142_eepa ACGCACGGTAGACAGACTAGCAACGACTATCG(+AAAAAA)GTACCATCTCCACTCCTGTCATCTCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606114140_vtd2 TCGTGGACATGGCAAAACCAGGCAAGAGCCAG(+AAAAAA)GATCCACAGAGAAACCCTCTTGCTTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606114139_yh3b CCCAGAGAAGCAAGCAAGAGGGTTTCTCTGTG(+AAAAAA)GATCCTGGCTCTTGCCTGGTTTTGCCATGTC NGG 10 8 17 8 24 0 100 5 10